Reduced impact of pyrimethamine drug pressure on Plasmodium malariae 1 dihydrofolate reductase
نویسندگان
چکیده
8 1 Unité d’Epidémiologie Moléulaire du Paludisme, Institut Pasteur du Cambodge, Phnom Penh, Cambodge 9 2 Génopôle de l'Ile de France, Plate-forme Génomique, Institut Pasteur, Paris, France 10 3 Unité de Parasitologie Médicale, Centre International de Recherches Médicales de Franceville, Franceville, 11 Gabon 12 4 Centre de Recherches Médicales et Sanitaires, CERMES, Niamey, Niger 13 5 Departement de Santé Publique, Faculté de Médecine, Université d’Antananarivo, Antananarivo, Madagascar 14 6 Laboratoire de Parasitologie-Mycologie, AP-HP Hôpital Avicenne, Bobigny, France 15
منابع مشابه
Genetic mutations in 57 and 58 codons gene of Plasmodium vivax dihydrofolate reductase
Introduction: The use of Sulfadoxine and pyrimethamine (SP) for treatment of vivax malaria is not common in most of malarious areas because of sensivity of this parasite to chloroquine. But, Plasmodium vivax isolates are exposed to SP because of mixed infection with P.falciparum and this subject has lead to emergence of mutations in P.vdhfr gene. As Plasmodium vivax is the most prevalent specie...
متن کاملGenetic analysis of the dihydrofolate reductase-thymidylate synthase gene from geographically diverse isolates of Plasmodium malariae.
Plasmodium malariae, the parasite responsible for quartan malaria, is transmitted in most areas of malaria endemicity and is associated with significant morbidity. The sequence of the gene coding for the enzyme dihydrofolate reductase-thymidylate synthase (DHFR-TS) was obtained from field isolates of P. malariae and from the closely related simian parasite Plasmodium brasilianum. The two sequen...
متن کاملRapid selection of Plasmodium falciparum dihydrofolate reductase mutants by pyrimethamine prophylaxis.
A prospective study was conducted to measure the selective effect of pyrimethamine prophylaxis on point mutations in Plasmodium falciparum dihydrofolate reductase (DHFR). A total of 109 Malian children were given pyrimethamine weekly for 5 weeks. P. falciparum infections were analyzed by polymerase chain reaction for DHFR mutations, which were dramatically more frequent among prophylaxis-breakt...
متن کاملQuadruple mutations in dihydrofolate reductase of Plasmodium falciparum isolates from Car Nicobar Island, India.
Quadruple mutations in the Plasmodium falciparum dihydrofolate reductase (PFDHFR) enzyme give rise to the highest level of pyrimethamine resistance leading to treatment failures. We describe here the presence of these quadruple mutations in a majority of P. falciparum isolates from Car Nicobar (Andaman and Nicobar) Island, India. Isolates from the mainland, however, continue to show a prevalenc...
متن کاملPlasmodium falciparum strains harboring dihydrofolate reductase with the I164L mutation are absent in Malawi and Zambia even under antifolate drug pressure.
The Plasmodium falciparum dihydrofolate reductase (PfDHFR) enzyme is the target of pyrimethamine, a component of the antimalarial pyrimethamine-sulfadoxine. Resistance to this drug is associated primarily with mutations in the Pfdhfr gene. The I164L mutant allele is of particular interest, because strains possessing this mutation are highly resistant to pyrimethamine and to chlorproguanil, a co...
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